https://journals.psmpublishers.org/index.php/microbiol/issue/feed PSM Microbiology 2018-02-21T22:38:02+00:00 PSM Microbiology microbiol@psmpublishers.org Open Journal Systems <p style="text-align: justify;">PSM Microbiology (ISSN: 2518-3834) is a peer-reviewed, multidisciplinary, open access,&nbsp; international journal that considers articles on all aspects of microbiology and allied sciences.</p> https://journals.psmpublishers.org/index.php/microbiol/article/view/144 Anti-Candida activity of cinnamon Inhibition of virulence factors of clinical strains of Candida albicans by essential oil of Cinnamomum zeylanicum 2018-02-21T22:38:02+00:00 Priscila Cordeiro de Lima Carvalho niveasap@gmail.com Nivea Pereira de Sá niveasap@gmail.com Inayara Cristina Alves Lacerda niveasap@gmail.com Carla Pataro niveasap@gmail.com Luiz Henrique Rosa lhrosa@icb.ufmg.br Rodrigo Silva Alves niveasap@gmail.com Juliana Pereira Lyon niveasap@gmail.com Carlos Augusto Rosa carlrosa@icb.ufmg.br Susana Johann sjohann@icb.ufmg.br <p>Essential oil of cinnamon (<em>Cinnamomum zeylanicum)</em> has been used in medicine and food additives. In the present work, we examined the antifungal activity of essential oil extracted from <em>C. zeylanicum</em> bark against oral opportunistic strains of <em>Candida</em> <em>albicans</em>. In addition, we verified the capability of this essential oil to inhibit fungal adhesion to buccal epithelial cells (BECs), germ tube formation, and proteinase activities. Cinnamon oil presented minimum inhibitory concentration (MIC) values that ranged from 31.2 to 125 µg mL<sup>-1 </sup>against the isolates of the tested <em>C. albicans</em> strains. Cinnamon essential oil could inhibit the adhesion of <em>C. albicans</em> to BECs for all of the isolates tested in present study. The <em>C. albicans</em> strains showed inhibited proteinase production after treatment with cinnamon oil, based on the MIC values determined for each <em>C. albicans </em>strain. Cinnamon oil was also able to inhibit the germ tube formation of all isolates of <em>C. albicans</em>, with the percentage of inhibition ranging from 44.7 to 82.9%. Our results showed that cinnamon oil presented potent antifungal activity and the ability to inhibit virulence factors of oral pathogenic strains of <em>C. albicans</em>.</p> 2018-02-21T22:10:07+00:00 ##submission.copyrightStatement## https://journals.psmpublishers.org/index.php/microbiol/article/view/158 Determination of Cutoff of IgM ELISA for Diagnosis of Scrub typhus in Hilly Northern State of Himachal Pradesh 2018-02-13T21:23:55+00:00 Anshu Gupta dranshu89@gmail.com <p>The aim of the study was to determine geographically relevant cutoffs for IgM ELISA in Himachal Pradesh. A total of 392 individuals including 292 patients of all age groups with clinically suspected scrub typhus and 100 healthy people were enrolled after taking a proper consent at Indira Gandhi Medical College, Shimla. An indirect ELISA that detects IgM antibodies to&nbsp;<em>Orientia tsutsugamushi</em>&nbsp;was performed in all serum samples. The serum samples from patients were subjected to IgM immunofluorescent antibody (IFA) (Fuller Laboratories, USA). A receiver operating characteristic (ROC) curve was drawn for ELISA to generate cutoff for the test. A total of 192 patients were found to be positive for scrub typhus. A set of 100 sera samples collected from healthy volunteers from Shimla were used to establish region specific cutoff for IgM ELISA. The cut off calculated from healthy volunteer was mean OD +3 standard deviation = 0.5. The ROC curve analysis of 392 participants revealed a cutoff-OD value of 0.46 with sensitivity and specificity of 91.7% and 99.5% respectively. Hence, a cutoff-OD value of 0.46 or 0.5 can be used as the cutoff for the diagnosis of scrub typhus for Himachal Pradesh based on our findings.</p> 2018-02-13T00:00:00+00:00 ##submission.copyrightStatement##