Incidence of Antibiotic Resistant Coliforms in Poultry Meat in Menoufia Governorate, Egypt

Authors

  • Reyad R. Shawish Department of Food Hygiene & Control, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32958, Egypt.
  • Abd-Rahman M. El-Bagory Department of Food Hygiene & Control, Faculty of Veterinary Medicine, Menofia University, Menofia 32958, Egypt.
  • Shimaa Samir Elnahriry Department of Bacteriology, Mycology, and Immunology, Faculty of Veterinary Medicine, University of Sadat City, Menofia 32958, Egypt.
  • Heba A. Wafy Directorate of Veterinary Medicine, El-Menofia Governorate, Egypt.
  • Haitham Helmy Sayed Department of Microbiology, Faculty of Veterinary Medicine, Souhag University, Souhag, 82524, Egypt.

Keywords:

Antimicrobial resistance, coliforms, chicken meat, Egypt.

Abstract

A total of 100 random samples of fresh chicken cuts (50 each of breast and thigh) were collected from different butcher’s shops at Menoufia Governorate. The samples were examined for the coliform count, isolation and identification of coliform strains, and antibiotic sensitivity. The obtained results indicated that the coliform count varied from 2.7x102 to 9.1x104 CFU/g for chicken thigh and 2.3x102 to 6.3x104 CFU/g for chicken breast respectively. Coliform isolates were identified as Escherichia coli, Citrobacter freundii, Citrobacter diversus, Enterobacter agglomerans, Enterobacter aerogenes, Enterobacter cloacae, Enterobacter hafniae, Serratia liquefaciens, Klebsiella pneumoniae and Klebsiella ozaenae. Moreover, the serological examination of E. coli isolates revealed E. coli O86: K6, E. coli O55: K59, E. coli O125: K70, E. coli O128: K67, E. coli O26: K60 and E. coli O111: K58. Antimicrobial resistance among isolated coliform strains was detected by susceptibility to 8 antibiotics by disc diffusion method. Generally, E. coli isolates were resistant to ciprofloxacin, vancomycin, norfloxacin, streptomycin, and kanamycin. C. freundii isolates were resistant to ciprofloxacin, vancomycin, erythromycin, cefoxitin, and norfloxacin; E. agglomerans isolates were resistant to vancomycin, erythromycin, cefoxitin, streptomycin, and kanamycin. E. aerogenes isolates were resistant to vancomycin, cefoxitin, and kanamycin. E. cloacae and K. ozaenae strains were resistant to ciprofloxacin, norfloxacin, and kanamycin. S. liquefaciens strains were resistant to ciprofloxacin, vancomycin, cefoxitin, norfloxacin, and kanamycin. K. pneumonia strain was sensitive to all tested antibiotics. These data suggest that antimicrobial-resistant coliform isolates are widely distributed in the meat and processing environment in Egypt, which can play a role in the dissemination of antimicrobial resistance to other pathogenic and commensal bacteria.

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Published

2020-03-31

How to Cite

Shawish, R. R., El-Bagory, A.-R. M., Elnahriry, S. S., Wafy, H. A., & Sayed, H. H. (2020). Incidence of Antibiotic Resistant Coliforms in Poultry Meat in Menoufia Governorate, Egypt. PSM Microbiology, 5(1), 7-13. Retrieved from https://journals.psmpublishers.org/index.php/microbiol/article/view/412

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