Rice Chitinase Gene Expression in Genetically Engineered Potato Confers Resistance against Fusarium solani and Rhizictonia solani

  • Madiha Zaynab College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Sonia Kanwal Institute of Horticulture Sciences, University of Agriculture, Faisalabad 38040, Pakistan.
  • Iqbal Hussain National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Muhammad Qasim College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Ali Noman College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Umer Iqbal National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Ghulam Muhammad Ali National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Khalida Bahadar National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Aatka Jamil National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Kalsoom Sughra Department of Agronomy, Sindh Agricultural University, Tando jam, Sindh Pakistan.
  • Nazia Rehman National Agriculture Research Centre, Islamabad 44000, Pakistan.
  • Mahmooda Buriro Department of Agronomy, Sindh Agricultural University, Tando jam, Sindh Pakistan.
  • Safdar Abbas Department of Biochemistry, Quid-e-Azam University, Islamabad, Pakistan.
  • Mohsin Ali Department of Plant Pathology, University of Agriculture, Faisalabad 38040, Pakistan.
  • Anwaar Hayder Alvi Institute of Molecular Biology & Biotechnology, Bahauddin Zakariya University, Multan, Pakistan.
  • Muhammad Anwar College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Muhammad Ifnan Khan College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
  • Muhammad Tayyab College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Keywords: Dry rot, Fusarium wilt, Tissue culture, Transgenic, Vector.

Abstract

The present study was planned to evaluate the transgenic potato plants containing chitinase (RCG-3) gene transformed through Agro bacterium for resistance against Rhizoctonia Solani and Fusarium solani. In vitro, plants were cultured for multiplication and then pathogenicity of transgenic potato plants harboring chitinase gene were carried out with fungal pathogens (R. solani and F. solani). The fungal pathogens were isolated from infected tubers and purified by single spore technique. For multiplication of pathogens wheat straw method was used for R.solani and sorghum seed method was used for F.solani. The inoculation of fungal infection (R. solani) and (F. solani) transgenic and non-transgenic plants were performed in laboratory and controlled greenhouse conditions (Containment). Results showed that transgenic potato plants containing chitinase (RCG-3) gene transformed through Agrobacterium showed resistance against R. Solani and F. solani compared to non-transgenic plants. It was concluded that rice chitinase gene expression in potato confers enhanced resistance against two major fungal diseases of potato in Pakistan.

Published
2017-11-02
How to Cite
Zaynab, M., Kanwal, S., Hussain, I., Qasim, M., Noman, A., Iqbal, U., Ali, G., Bahadar, K., Jamil, A., Sughra, K., Rehman, N., Buriro, M., Abbas, S., Ali, M., Alvi, A., Anwar, M., Khan, M., & Tayyab, M. (2017). Rice Chitinase Gene Expression in Genetically Engineered Potato Confers Resistance against Fusarium solani and Rhizictonia solani. PSM Microbiology, 2(3), 63-73. Retrieved from https://journals.psmpublishers.org/index.php/microbiol/article/view/146
Section
Articles