Isolation and Identification of Protease Enzyme Producing Bacillus subtilis from Soil

  • Fakhar-ul-Nisa Yunus Department of Zoology, Lahore College for Women University, Jail Road Lahore 54000, Pakistan.
  • Husna Malik Department of Zoology, Lahore College for Women University, Jail Road Lahore 54000, Pakistan.
  • Saira Hadi Department of Zoology, Lahore College for Women University, Lahore, 54000, Pakistan.
  • Mirza Imran Shahzad University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Bahawalpur 63100, Pakistan; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
  • Muhammad Irfan Department of Biochemistry and Molecular Biology, University of Gujrat, HH Campus, Gujrat, Pakistan.
  • Shahzad Ali Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore, Pakistan.
  • Ali Muhammad Department of Zoology, University of Poonch, Rawalakot 12350, Azad Kashmir, Pakistan.
Keywords: Bacillus subtilis, industrial areas of Lahore, spores, zone formation test, protease enzyme, soil.

Abstract

Bacillus subtilis is commonly recovered from water, soil, air and decomposing plant residue. But the main habitat of B. subtilis is soil and a long term survival is expected to occur in soil. In this study twenty samples of soil were collected from different industrial areas of Lahore. These soil samples were used to analyze the presence of microorganisms in it. Bacteria were grown on the specific medium for the colony identification. Then Biochemical test was performed to isolate and identify B. subtilis from the soil samples. In the present study, Bacillus species were found to be abundant in our soil samples. The bacteria isolated from the soil were rod shaped gram positive bacteria that form spores under stress conditions. The spores were appeared to be rounded and green in color inside the cell. Positive zone formation test confirmed the presence of B. subtilis. The protease enzyme was produced by B. subtilis in large quantities during vegetative growth. It was found that absorbance reached to its maximum value at 5µg/ml concentration of protease enzyme. The maximum values of absorbance were 0.45 and 0.5 at 550nm. It was concluded from the above results that soil is the main habitat of many microorganisms and among these microorganisms Bacillus subtilis was found to produce protease enzyme.

Published
2017-03-30
How to Cite
Yunus, F.- ul-N., Malik, H., Hadi, S., Shahzad, M. I., Irfan, M., Ali, S., & Muhammad, A. (2017). Isolation and Identification of Protease Enzyme Producing Bacillus subtilis from Soil. PSM Microbiology, 2(1), 15-19. Retrieved from https://journals.psmpublishers.org/index.php/microbiol/article/view/112
Section
Articles