International Journal of Nanotechnology and Allied Sciences <p>International Journal of Nanotechnology and Allied Sciences (IJNAS; ISSN: 2523-9252) is a peer-reviewed, open access,&nbsp; international journal that considers articles on all aspects of nanotechnology and allied sciences.</p> en-US (International Journal of Nanotechnology and Allied Sciences) (MANI MUGHAL) Fri, 31 May 2019 00:00:00 +0000 OJS 60 Antioxidant Capacity of In-vitro generated Solanum tuberosum L. Cultivar (Favorita) <p>Plant-based diets are rich in antioxidants that scavenge harmful free radicals in order to reduce the risk of the development of oxidative stress-related diseases. <em>Solanum tuberosum</em> L. is endowed with a high content of antioxidants including phenolic acids and flavonoids, however, concentrations of these secondary metabolites in cultivars such as Favorita remain largely undefined. Thus, the total phenolic contents of Favorita were analyzed using HPLC, while percentage inhibition of DPPH and ABTS assays were performed to determine antioxidant activities. The major constituents of phenolic acid in the potato root methanol extract were vanillic acid and chlorogenic acid. Similarly, antioxidant activity ranged from 5.7% (% inhibition of DPPH) to 69.85% (% inhibition of ABTS) in comparison to the three available standards. Total phenolic content and antioxidant capacity revealed a significantly high linear correlation (R<sup>2 </sup>= 0.951, <em>P</em> = 0.01), suggesting a direct involvement of phenolics in the antiradical activity. These results demonstrate that Favorita contains potent antioxidant capacity; however, this potency is dependent on the specific free radicals utilized. These findings would facilitate future research on potato micropropagation for improved agronomic traits as well as give information on its nutritional use as an antioxidant.</p> Mehtab Muhammad Aslam, Karanja J. K., Suleiman Kehinde Bello, Madiha Zaynab, Mahpara Fatima, Junaite Bin Gias Uddin Copyright (c) 2019 PSM Fri, 31 May 2019 00:00:00 +0000 PCR and Nanotechnology Unraveling Detection Problems of the Seed-borne Pathogen Cephalosporium maydis, the Causal Agent of Late Wilt Disease in Maize <p>Late wilt disease of maize caused by the fungus <em>Cephalosporium maydis </em>is one of the most important fungal diseases in Egypt. The pathogen spreads through the remaining plant debris in soil and through apparently healthy seeds. Detection of the latent infection of <em>C. maydis </em>pathogen is of great importance step in disease management which commonly achieved by applying the standard seed health tests. The commonly used seed health tests in laboratories around the world are still lake of specificity, sensitivity, speed, simplicity, cost-effectiveness, and reliability of seed health methods. In the current study, Infection percentage of seed-borne <em>C. maydis</em> recorded 1.0 and 1.5% in 2015 and 2016, respectively from whole maize seed (Giza 2) when examined by the standard blotter method. Using the same detection method examining broken maize seeds of cv. Giza 2 gave 3.5 and 5% in 2015 and 2016, respectively. Maximum infection percentage obtained during the current study period was 6.5% which was gotten when Potato Dextrose Yeast Agar (PDYA) applied with broken maize seeds of the same cultivar. While the highest infection percentage recorded during the entire study was 11% which obtained from broken seeds of hybrid Sc 166 of season 2015 and incubated on PDYA. Relatively to other major fungi recorded, the deep freezing method used with whole seeds (Giza 2) gave the least records of <em>C. maydis</em> in 2015 and 2016; 0.0 and 1.2, respectively. On the other hand, PDYA method gave the highest record during the two successive years of investigation (18.2) obtained from 2016 broken seeds. PCR has many beneficial characteristics that make it highly applicable for detecting <em>C. maydis </em>from seeds. In PCR diagnosis study, the development of DNA extraction is one of the most important steps. These approaches include using Ag NPs and Fe NPs in DNA extraction method to enhance the quantity and purity of the DNA template for successful PCR assay.</p> Ahmed Mohamed Awad, Ibrahim Hassan El-Abbasi, Tahsin Shoala, Sahara Ahmed Youssef, DaliaMohamed Shaheen, Gomaa Ahmed Amer Copyright (c) 2019 PSM Fri, 31 May 2019 00:00:00 +0000