International Journal of Nanotechnology and Allied Sciences https://journals.psmpublishers.org/index.php/ijnas <p>International Journal of Nanotechnology and Allied Sciences (IJNAS; ISSN: 2523-9252) is a peer-reviewed, open access,&nbsp; international journal that considers articles on all aspects of nanotechnology and allied sciences.</p> PSM en-US International Journal of Nanotechnology and Allied Sciences 2523-9252 Phytochemical Screening, Antimicrobial, Antioxidant and Anticancer Activities of Yemeni Croton socatranus https://journals.psmpublishers.org/index.php/ijnas/article/view/608 <p><em>Croton socatranus</em> is a shrub or small tree endemic to Socotra Island and is widespread on the island. It has some interesting ethnomedicinal uses. The dry powder was subjected to extraction till exhaustion using methanol. The methanol extract was evaluated phytochemically, chromatographically, and for its antimicrobial and antioxidant activity. Toxicological and cytotoxic effects were also investigated. Microscopic examination of <em>C. socatranus</em> aerial parts confirmed the presence of xylem vessels, sclerenchymatous cells, slender-shaped fibers, vessel elements with scalariform thickenings, palisade cells, paracytic stomata, and stellate trichomes. Phytochemical investigation showed that the methanol extract possessed alkaloids, high levels of phenolic compounds, tannins, flavonoids, triterpenes, diterpenes, phytosterol, and saponin. Notably, the methanol extract of <em>C. socatranus</em> did not cause any mortality or behavioral changes in the rats even at the highest tested dose (5000mg/kg of methanol extract). It showed inhibition zone against <em>Streptococcus pneumonia </em>and <em>Escherichia coli </em>and MIC values were between 156.25- 655 µg/ml for gram-positive strains while 625-2500 µg/ml for gram-negative bacteria. Antifungal activity was recorded against ten tested fungal species. Furthermore, a dose-dependent antioxidant activity was observed. <em>C. socatranus </em>extract displayed moderate cytotoxic activity against eight cancerous cell lines. The extract was highly effective on MCF-7, HepG-2, and RD cell lines when compared with other cell lines and the IC50 was less than 30 µg/ml which suggests the presence of selective cytotoxic compounds. Further investigations are required to understand the possible mechanism(s) of action of this extract on various cancer cells and the isolation of active phytochemicals.</p> Ibrahim Ali Al-Hakami Amina El-Shaibany Hassan Al-Mahbashi Abdulrahman Humaid Salwa Raweh Mahmoud M. Elaasser Copyright (c) 2022 PSM https://creativecommons.org/licenses/by-nc/4.0/ 2022-03-03 2022-03-03 6 1 1 13 Fabrication and Characterization of Antibacterial Biodegradable Polymeric Nanofibers of Polyvinyl Alcohol loaded with Levofloxacin for External Skin Infection https://journals.psmpublishers.org/index.php/ijnas/article/view/643 <p>Electrospun nanofibrous membranes have gained great focus in medical research due to its biocompatibility and biodegradability. Research proposed that it is most advanced method of delivering drug to patient’s body at a particular site of infection. Through this targeted delivery, there is increased rate of delivered drug to an infected body part; it may be organs, tissues or cells. As result, the action of recovery of infection is enhanced without causing any side effects. During present study sustainable electrospun nanofibers were fabricated via biodegradable synthetic polymer polyvinyl alcohol (PVA) loaded with antibiotic (Levofloxacin). Control (PVA) and antibiotic loaded (PVA/LVF) nanofibers were synthesized through simple electrospinning. Synthesized materials were characterized through scanned electron microscopy (SEM) for morphology that showed average fiber diameter size of 146.24±44.024 µm and 184.79±41.94 µm respectively. While for chemical characterization FTIR was carried out. Further antibacterial susceptibility was checked against <em>Escherichia coli </em>for different time period of incubation and maximum zone of inhibition was observed at 72 hours that was 29±0.25 mm as compared to 24 h and 48 h. While release rate of drug in artificial medium phosphate buffer saline (PBS) was measured by spectrophotometric method up to 72 hours. In the medium at initial stage nano fibers showed burst release of drug up to 24 hour, later on sustain release behavior was observed up to 72 h. From the findings it was suggested that synthesized material especially used in the synthesis of gauze for external skin infections, it could also be used in the synthesis of sutures, wound patches bandages and other biomedical applications.</p> Ghulam Murtaza Khuhro M. Aqeel Bhutto Adeela Chachar Mahesh Kumar Copyright (c) 2022 PSM https://creativecommons.org/licenses/by-nc/4.0/ 2022-05-31 2022-05-31 6 1 14 22