Characterization of Ceftazidime Resistance in Clinical Isolates of Bacteria in Lahore, Pakistan

  • Mehwish Saleem Department of Zoology, Govt.Post Graduate Islamia College (W) Cooper Road, Lahore, Pakistan; Microbiology Section, Sir ganga Ram Hospital, Lahore, Pakistan.
  • Ayesha Batool Department of Zoology, Govt.Post Graduate Islamia College (W) Cooper Road, Lahore, Pakistan; Microbiology Section, Sir ganga Ram Hospital, Lahore, Pakistan.
  • Muhammad Naeem Iqbal The School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
  • Asfa Ashraf The School of Life Sciences, Fujian Normal University, Fuzhou 350117, China; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
Keywords: Ceftazidime, antibiotic sensitivity, bacteria, Sir Ganga Ram Hospital.

Abstract

The aim of the current study was to determine Ceftazidime resistance in clinical isolates of bacteria in Lahore, Pakistan. This study was carried out at Lahore in Pathology Laboratory, Sir Ganga Ram Hospital from January to June 2018.  In order to study the Ceftazidime resistant pattern, a total of 190 clinical samples were collected from different patients. From these collected samples exceeding number 88 were of pus samples followed by 56 blood, 25 sputum and 21 urine samples. All clinical samples were subjected to conventional cultural and biochemical methods for microbial enumeration. Antibiotic sensitivity was analyzed using agar disk diffusion method. The results revealed a higher prevalence of clinical samples among females (53.68%), and in age group 0-20 years (67%). Prevalence of bacteria isolated was Escherichia coli (81.57%), followed by Staphylococcus aureus (7.89%), Pseudomonas sp. (4.22%), Klebsiella sp. (3.16%), and Proteus sp. (3.16%). Rate of resistance to Ceftazidime was higher in females than in the males. Teenagers were highly resistant to Ceftazidime. Among the bacterial isolates, higher resistance to Ceftazidime was shown by E.coli (56.31%), followed by S.aureus (6.84%), Pseudomonas sp. (4.21%), Klebsiella sp. (1.57%), and least resistance by Proteus sp. (1.05%). Resistance to Ceftazidime illustrated that more consumption of a specific antibiotic leads to sustainability of resistance against those antibiotics.

Published
2018-10-26
How to Cite
Saleem, M., Batool, A., Iqbal, M., & Ashraf, A. (2018). Characterization of Ceftazidime Resistance in Clinical Isolates of Bacteria in Lahore, Pakistan. International Journal of Molecular Microbiology, 1(2), 44-50. Retrieved from https://journals.psmpublishers.org/index.php/ijmm/article/view/234
Section
Articles