Fluorescence Activated Cell Sorting (FACS): An Advanced Cell Sorting Technique

  • Ammara Naeem Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
  • Noreen James Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
  • Misfa Tanvir Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
  • Madiha Marriam Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
  • Sammar Nathaniel Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
  • Sammar Nathaniel Department of Biochemistry, Kinnaird College for Women, Lahore, Pakistan.
Keywords: Fluorescence-activated cell sorting (FACS); signal detection and amplification; proteins; stem cells; nucleic acid

Abstract

Fluorescence-activated cell sorting (FACS) follows the principle of flow cytometry. It is used to separate the molecules or cells of interest from the heterogeneous mixture by selecting the specific cells. The sample is prepared by labelling the cells with fluorescence dye. It is excited by a laser source which results in scattering and fluorescence due to absorbed light. A number of systems have been produced using two or three lasers to excite several dyes. The light scatter and fluorescence detector outputs are amplified and processed for evaluation. The function of fluorescent excitation and detection system is to produce the signals which accurately reflect the amount of each dye associated with the cell. It is applied in the biological research field, i.e. lymphocyte determination, isolation and characterization of infected stem cells, screening of protein binding with various ligands and for the quantification of nucleic acid content in a cell.

Published
2017-05-01
How to Cite
Naeem, A., James, N., Tanvir, M., Marriam, M., Nathaniel, S., & Nathaniel, S. (2017). Fluorescence Activated Cell Sorting (FACS): An Advanced Cell Sorting Technique. PSM Biological Research, 2(2), 83-88. Retrieved from https://journals.psmpublishers.org/index.php/biolres/article/view/65
Section
Review Articles