A Comparative Study on Isolation and Identification of Bacillus thuringiensis from Different Localities of Gujranwala City

  • Fakhar-un-Nisa Yunus Department of Zoology, Lahore College for Women University, Lahore 54000, Pakistan.
  • Faiza Kanwal Department of Zoology, Lahore College for Women University, Lahore 54000, Pakistan.
  • Farzana Rashid Department of Zoology, Lahore College for Women University, Lahore 54000, Pakistan.
  • Asfa Ashraf Department of Zoology, Lahore College for Women University, Lahore 54000, Pakistan; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
  • Muhammad Naeem Iqbal The School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
  • Shiwei Xiao The School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Keywords: Bacillus thuringiensis, Cry proteins, SDS-PAGE, biopesticide

Abstract

This was a comparative study on isolation and identification of Bacillus thuringiensis isolates from different localities of city Gujranwala. Isolates of B. thuringiensis were identified using microscopic characters, colony morphology and biochemical characters. Then SDS-PAGE was performed to obtain a high protein profile on the basis of Cry proteins. A total of 74 out of 100 samples were found positive with B. thuringiensis. The maximum diversity of isolates was present in the soil (25%) as compared to other samples like dust (23.8%), cow dung (17.5%), donkey dung (17.5%) and bird droppings (16.2%). The average value of bacterial load was highest in soil (1 ± 0.192 CFU/ml) and lowest in bird droppings (0.650 ± 0.131 CFU/ml). The different sizes of bands with representative nine isolates from all sample sources on SDS-PAGE represent characteristics of crystal proteins having different target order specificity. It was concluded that B. thuringiensis is prevalent in various localities of the city. The need of the hour is to characterize these isolates and use them for pesticidal activity.

Published
2016-05-14
How to Cite
Yunus, F.- un-N., Kanwal, F., Rashid, F., Ashraf, A., Iqbal, M. N., & Xiao, S. (2016). A Comparative Study on Isolation and Identification of Bacillus thuringiensis from Different Localities of Gujranwala City. PSM Biological Research, 1(1), 34-38. Retrieved from https://journals.psmpublishers.org/index.php/biolres/article/view/20
Section
Articles