In Vitro Improved Production of Monoclonal Antibody against Zearalenone in Supplemented Cell Culture Media

  • Muhammad Naeem Iqbal Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan.
  • Asfa Ashraf Pakistan Science Mission (PSM), Noor Kot 51770, Pakistan; The School of Life Sciences, Fujian Normal University, Fuzhou 350117, China.
  • Sumei Ling Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
  • Shihua Wang Key Laboratory of Pathogenic Fungi and Mycotoxins of Fujian Province, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Keywords: Monoclonal antibody, zearalenone, hybridoma technology.

Abstract

Zearalenone (ZEN) is a phenolic resorcylic acid lactone, produced by several Fusarium species causing reproductive disorders such as abortion and stillbirth in livestock resulting from hyperestrogenism. A monoclonal antibody (mcAb) specific to zearalenone was produced from hybridoma cell line 5A8. It was generated by the fusion of Sp2/0 myeloma cells with spleen cells isolated from Balb/c mice with highest-titer (1: 64,000, v/v). The mice were immunized intraperitoneally with zearalenone–cationic bovine serum albumin (ZEN-cBSA). ZEN-cOVA conjugate was used as coating antigen to test titer of mice serum. Hybridoma cells were grown on cell culture media supplemented with carbohydrates (CHO) respectively. Monoclonal antibody produced by hybridoma in vitro belongs to the immunoglobulin subtype IgG1 with specific antibody titer 0.70. The most effective culture medium for hybridoma clones was supplemented with maltose that achieved best titer at 1.485 as determined by icELISA. The use of supplemented cell culture media is an effective approach for enhanced production of monospecific-antibody, better hybridoma growth and viability.

Published
2018-08-17
How to Cite
Iqbal, M., Ashraf, A., Ling, S., & Wang, S. (2018). In Vitro Improved Production of Monoclonal Antibody against Zearalenone in Supplemented Cell Culture Media. PSM Biological Research, 3(3), 106-110. Retrieved from http://journals.psmpublishers.org/index.php/biolres/article/view/209
Section
Articles